surehyb-enabled hybridization chamber (Agilent technologies)
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Agilent technologies
surehyb-enabled hybridization chamber
Surehyb Enabled Hybridization Chamber, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/surehyb+chambers/pm38203750-383-18-17
Average 90 stars, based on 1 article reviews
Surehyb Enabled Hybridization Chamber, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/surehyb+chambers/pm38203750-383-18-17
Average 90 stars, based on 1 article reviews
surehyb-enabled hybridization chamber - by Bioz Stars,
2026-10
90/100 stars
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Incubation:Article Title: Curcumin is a potent modulator of microglial gene expression and migration Article Snippet: Briefly, 200 ng of total RNA were labeled with Cy3 using the Agilent Quick-Amp Labeling Kit - 1 color according to the manufacturer's instructions. cRNA was purified with the RNeasy Mini Kit (Qiagen) and labeling efficiency was determined with a NanoDrop ND-1000 photometer (PeqLab). .. The arrays were incubated with cRNAs in Hybridization:Article Title: Th17 cells target the metabolic miR-142-5p-SDHC/SDHD axis promoting invasiveness and progression of cervical cancers Article Snippet: The purification of the labeled cDNA was performed with RNeasy Mini kit (Qiagen) according to Agilent’s One-Color Gene Expression Microarray Protocol followed by the quantification using a NanoDrop2000 Spectrophotometer (Thermo Fisher Scientific). .. The hybridization of 600 ng labeled cRNA to the microarray slide was carried out at 65°C, 17 hours, 10 rpm in the Article Title: Assessing the Link between Diabetic Metabolic Dysregulation and Breast Cancer Progression Article Snippet: Finally, the labelled cRNA samples were fragmented at 60 °C and hybridized onto an Agilent Human Gene Expression Microarray 8 × 60 K. The Gene Expression Hybridization kit (Agilent Technologies, in situ Hybridization kit, Part Number 5190-0404) fragmented the labelled cRNA. .. Next, hybridization was performed in Agilent’s Article Title: Th17 cells target the metabolic miR-142-5p-succinate dehydrogenase subunit C/D (SDHC/SDHD) axis, promoting invasiveness and progression of cervical cancers. Article Snippet: The purification of the labeled cDNA was performed with RNeasy Mini kit (Qiagen, Hilden, Germany) according to Agilent’s One-Color Gene Expression Microarray Protocol followed by the quantification using a NanoDrop2000 Spectrophotometer (Thermo Fisher Scientific, Schwerte, Germany). .. The hybridization of 600 ng labeled cRNA to the microarray slide was carried out at 65 °C, 17 h, 10 r.p.m. in the Article Title: Influence of cell cycle on responses of MCF-7 cells to benzo[a]pyrene Article Snippet: Agilent Human 4 × 44K Genome-wide arrays were used and the reference design was applied, whereby a Universal Human Reference RNA (Stratagene, La Jolla, USA) was hybridised to every sample. .. Cy3-(sample)- and Cy5-(reference)-labelled probes were hybridised to the oligo microarrays using the Gene Expression Hybridization Kit (Agilent Technologies, UK) using Labeling:Article Title: Th17 cells target the metabolic miR-142-5p-SDHC/SDHD axis promoting invasiveness and progression of cervical cancers Article Snippet: The purification of the labeled cDNA was performed with RNeasy Mini kit (Qiagen) according to Agilent’s One-Color Gene Expression Microarray Protocol followed by the quantification using a NanoDrop2000 Spectrophotometer (Thermo Fisher Scientific). .. The hybridization of 600 ng labeled cRNA to the microarray slide was carried out at 65°C, 17 hours, 10 rpm in the Article Title: MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells Article Snippet: One hundred ng of each total RNA aliquot were treated with calf intestine phosphatase (GE Healthcare, Chalfont St Giles, UK), denatured using DMSO (Sigma, St Louis, MO), and directly labeled with Cy3 using T4 RNA ligase (GE Healthcare). .. Labeled samples were hybridized to the miR array 8 × 15 k (G4470A) platforms in Article Title: Th17 cells target the metabolic miR-142-5p-succinate dehydrogenase subunit C/D (SDHC/SDHD) axis, promoting invasiveness and progression of cervical cancers. Article Snippet: The purification of the labeled cDNA was performed with RNeasy Mini kit (Qiagen, Hilden, Germany) according to Agilent’s One-Color Gene Expression Microarray Protocol followed by the quantification using a NanoDrop2000 Spectrophotometer (Thermo Fisher Scientific, Schwerte, Germany). .. The hybridization of 600 ng labeled cRNA to the microarray slide was carried out at 65 °C, 17 h, 10 r.p.m. in the Microarray:Article Title: Th17 cells target the metabolic miR-142-5p-SDHC/SDHD axis promoting invasiveness and progression of cervical cancers Article Snippet: The purification of the labeled cDNA was performed with RNeasy Mini kit (Qiagen) according to Agilent’s One-Color Gene Expression Microarray Protocol followed by the quantification using a NanoDrop2000 Spectrophotometer (Thermo Fisher Scientific). .. The hybridization of 600 ng labeled cRNA to the microarray slide was carried out at 65°C, 17 hours, 10 rpm in the Article Title: Assessing the Link between Diabetic Metabolic Dysregulation and Breast Cancer Progression Article Snippet: Finally, the labelled cRNA samples were fragmented at 60 °C and hybridized onto an Agilent Human Gene Expression Microarray 8 × 60 K. The Gene Expression Hybridization kit (Agilent Technologies, in situ Hybridization kit, Part Number 5190-0404) fragmented the labelled cRNA. .. Next, hybridization was performed in Agilent’s Article Title: Th17 cells target the metabolic miR-142-5p-succinate dehydrogenase subunit C/D (SDHC/SDHD) axis, promoting invasiveness and progression of cervical cancers. Article Snippet: The purification of the labeled cDNA was performed with RNeasy Mini kit (Qiagen, Hilden, Germany) according to Agilent’s One-Color Gene Expression Microarray Protocol followed by the quantification using a NanoDrop2000 Spectrophotometer (Thermo Fisher Scientific, Schwerte, Germany). .. The hybridization of 600 ng labeled cRNA to the microarray slide was carried out at 65 °C, 17 h, 10 r.p.m. in the Functional Assay:Article Title: Oxidative Nanopatterning of Titanium Surface Influences mRNA and MicroRNA Expression in Human Alveolar Bone Osteoblastic Cells Article Snippet: Briefly, 500 ng of the individual total RNA was employed to synthesize double-stranded cDNA and cyanine 3 (Cy3) CTP labeled complementary amplified RNA (cRNA) by means of the Agilent Linear Amplification Kit (Agilent), according to the manufacturer's instructions. .. By using Agilent human 4 × 44 K oligonucleotide microarrays (Agilent), cyanine-labeled complementary RNA was hybridized to microarrays in other:Article Title: Amyloid fibril-based thixotropic hydrogels for modeling of tumor spheroids in vitro. Article Snippet: Biomaterials mimicking extracellular matrices (ECM) for three-dimensional (3D) cultures have gained immense interest in tumor modeling and in vitro organ development.. Here, we introduce a new class of amyloid fibrilbased peptide hydrogels as a versatile biomimetic ECM scaffold for 3D cell culture and homogenous tumor spheroid modeling.. We show that these amyloid fibril-based hydrogels are thixotropic and allow cancer cell adhesion, proliferation, and migration. Gene Expression:Article Title: Assessing the Link between Diabetic Metabolic Dysregulation and Breast Cancer Progression Article Snippet: Finally, the labelled cRNA samples were fragmented at 60 °C and hybridized onto an Agilent Human Gene Expression Microarray 8 × 60 K. The Gene Expression Hybridization kit (Agilent Technologies, in situ Hybridization kit, Part Number 5190-0404) fragmented the labelled cRNA. .. Next, hybridization was performed in Agilent’s Article Title: Influence of cell cycle on responses of MCF-7 cells to benzo[a]pyrene Article Snippet: Agilent Human 4 × 44K Genome-wide arrays were used and the reference design was applied, whereby a Universal Human Reference RNA (Stratagene, La Jolla, USA) was hybridised to every sample. .. Cy3-(sample)- and Cy5-(reference)-labelled probes were hybridised to the oligo microarrays using the Gene Expression Hybridization Kit (Agilent Technologies, UK) using |